Gerard Rozing

You are invited to follow the latest news about my participation in conferences, symposia, events and my publications in the field of liquid phase separation science in this blog. If you have a comment or suggestion for me about a post, please feel free to use the comment function at the bottom of each post. For your convenience, I have started adding a "home" icon to each post. Just click on the icon to return to the home page.

Unfortunately, I had to turn off the mail function of this site because it was being abused. But most of you will know how to contact me anyway.

From 2017 to 2020, I was a co-organizer of the CE Forum. The aim was to bring together people involved in capillary electrophoretic separations in the DACH region to present and discuss their ongoing research and, in particular, the problems and questions they face. The CE Forum provides a platform for graduate students and postdocs to advance their scientific careers. It has been an annual meeting since 2009.

The Corona epidemic interrupted the series 2021-2023. On the initiative of a group of former doctoral students of Prof. Christian Neusüß, Aalen University of Applied Sciences, the CE Forum was re-established. It occurred on February 28 and 29 at the Aalen University of Applied Sciences. Prof. Neusüß arranged for the university to host the event and to provide the necessary accommodation and services. Details about the organizers and the program can be found on the CE-Forum website.

HPLC 2023 Banner

Given my involvement with the upcoming 51st International Symposium on High-Performance Liquid Phase Separations and Related Techniques in this blog, I have collected relevant documentation that has been prepared under my responsibility. Download copies of official public HPLC2023 documents here.

cover CEMS for proteomics and metabolomics

Capillary Electrophoresis coupled with mass spectrometric detection is among the most powerful analytical tools in life science research. Incepted through basic research, described on these web pages, the technique was successfully introduced on the market by Hewlett-Packard (now Agilent Technologies) in the nineties. Their triple-tube sprayer has proven to be and still is the most robust, reliable, and easy-to-use interface, meeting user requirements in terms of separation resolution and sensitivity of detection. These benefits were obtained by using a sheath solvent which lifted the flow rate of the eluent, background electrolyte with the separate sample components, to a µL/min level.

The dilution of the separated sample bands through the mixing of the eluent with the sheath solvent by a factor of 10-50 is on the downside of this approach. So-called "sheathless" CE/MS interfaces have been developed since, e.g., the "porous tip interface," and introduced on the market by Beckman-Coulter (now Sciex). On the other hand, attempts to reduce the dimensions of sheath flow IFs and "liquid junction" type IF have become commercially available for research.

Many of you know that I have been responsible for arranging the review of posters competing for the Best Poster Award in many issues of the HPLC Symposium series and other separation science symposia since 2008. My last involvement with the HPLC Symposium series was with HPLC 2019 Milano three years ago. Due to the COVID-19 pandemic, HPLC 2020 has been postponed for two years as is HPLC2021 Düsseldorf now in 2023.  


Capillary Electrophoresis has been around for the past 40 years. It is a universal and ultra-high efficiency separation of low to high molecular weight substances. Manifested by different operation modes, these are all executed in cylindrical (capillary) or rectangular (chip) conduits.

The market perspectives of CE as an instrumental analysis tool in the eighties were immense. But has this perspective become a reality? This question is difficult to answer. Many commercial instruments use capillary electrophoresis as the primary separation method of the sample, but they are not designated as CE instruments.

Therefore, I felt it appropriate to map the "Capillary Electrophoresis" field according to methodologies, instrumentation, and areas of application (markets). The overview is based on publicly accessible information, my background, and experiences with CE during my professional career in Hewlett-Packard and Agilent Technologies, and inferences I made from that.

*A redacted version of this manuscript can be found in the July issue of "The Analytical Scientist"  via the following link.

Best Poster Award @ MSB 2021

Almost a tradition but also in this issue of the MSB symposium series, I arranged the reviews and chaired the panel of reviewers to honor the best posters at the 37th International Symposium on Microscale Separations and Bioanalysis (eMSB2021) organized by Dr. Alexander Ivanov of Northeastern University together with associated co-chairs (see Registered participants can access all oral and poster presentations from this page.

The full set of slides that I prepared for the award ceremony, at last, is given below, and a list of the awardees.

graphical abstractMicropillar array columns for advancing nanoflow HPLC

For quite some time, I had the idea to write a manuscript to explain the µPAC technology developed by Prof. Gert Desmet and commercialized by PharmaFluidics. I intended to describe the fundamental and practical barriers of obtaining ultimate separation performance of particle-packed columns (UHPLC) or of open tubular liquid chromatography (OTLC). It was aimed the manuscript to be a tutorial review, with emphasis on explaining the principle technology of pillar array-based separation column and demanding benchmark applications to demonstrate advantages of µPAC separations, especially for nanoflow HPLC.

The manuscript is now available in an open-access version via this link.

In case you do not have access but want a copy of the manuscript for personal usage, contact me via this form.



Review on Capillary Iso-Electric Focusing (CIEF)

In late 2019 I was invited to submit a review on Capillary Iso-Electric Focusing for Wiley Series Encyclopedia of Analytical Chemistry. Since I am not so deeply involved in research in CIEF, and it was in the scope of the series, it was agreed that the manuscript could be tutorial-style, aimed to explain and help the readers to step into CIEF.


Capillary isoelectric focusing (CIEF) has proven to be a very versatile separation method for characterization, identification, purity determination, and quantitation of amphoteric substances such as peptides and proteins. CIEF has become an indispensable and invaluable analytical tool in proteomics research, in the development of new biotherapeutics, and in quality and process control of their manufacturing.
The method is based on arranging and separating proteins according to their isoelectric point (pI), allowing to determine changes in the charge state of proteins after structural modifications such as deamidation, oxidation, isomerization, N-terminus modification, disulfide formation, and glycosylation.
Modifications that change biochemical characteristics of biotherapeutics such as their isoelectric point (pI) reduce their safety and long-term stability, influence drug efficacy, and increase undesired immunogenicity.

Here is the connection to the manuscript in the series. You will need a Wiley account for the Encyclopedia to access the paper. In case you do not have access but need a copy of the manuscript for private usage please contact me via the form.


Tutorial @ MSB2021 on Capillary Iso-Electric Focusing (CIEF)

At the 37th issue of the Microscale Separations and Bioanalysis symposium, I had the privilege to present a tutorial lecture on Capillary Iso-Electric Focusing. 

Abstract. Since the inception of iso-electric focusing (IEF) as a high-resolution electrophoretic technique for the separation of amphoteric substances by Tiselius and Vestenberg, it has become a prime methodology for the separation of protein mixtures.

A Tribute to Prof. K-P Hupe, Scientist, and Entrepreneur

Along with receiving the Pittcon Heritage Award 2021, Agilent Technologies in Waldbronn invited me to deliver a comprehensive presentation of the scientific works and the entrepreneurial endeavors of Prof. Peter Hupe at the first Agilent Infinity Lab LC virtual scientific conference, on June 22 and 23. Details of this conference can be found at the following location Agilent Virtual Event.

Pittsburgh Conference Heritage Award for Peter Hupe

As you may have learned otherwise, Prof. K-P. Hupe was honored with the Pittcon Heritage Award from the Science History Institute in Philadelphia. The award was presented at this year's virtual Pittsburgh Conference, Monday, March 8, 2021 The submission has been prepared in a joint effort of Fred Strohmeier and myself and with help from many others like Ron Majors and Dieter Hoehn.

The video registration of the award ceremony can be found here. The first speaker's recording is mono audio. Please use a headset with two loudspeakers.

The Development of HPCE at Hewlett-Packard in the eighties and the nineties

 At this year's virtual Pittsburgh Conference, I was invited to contribute to the Thirty-Second James L. Waters Symposium – Capillary Electrophoresis with an oral presentation on the development of High-Performance Capillary Electrophoresis instrumentation at Hewlett-Packard in the eighties and nineties of the last century. There were three more talks in this symposium by Norman Dovichi, Bob Weinberger, and Jeff Chapman. Program information is not available online anymore.

You can view the registration of the presentation here.


ITPlogoFrom September 1 to 4, the 36th International Symposium on Electroseparations and Liquid Phase Separation techniques took place at the University Paul Sabatier in Toulouse. Please check the website at for details on the scientific program and activities.

Like at other conferences, I was invited to arrange the review of posters for the Best Poster Award and the review of Oral Presentations by a Young Scientist (≤ 35 years) Award. You can find some more details at and in the award presentation that is posted at this location. (be aware that depending on your browser settings, the document goes directly to your download folder and does not open). The picture on the next page shows all the awardees together with the symposium chairmen, the president of the AfSep, and me, chairman of the review boards

Chromatography Today is a magazine very much like LC.GC, publishing manuscripts on new developments in the field of gas and liquid phase separations. Manuscripts may be logo chromatography todaycommunications on the application of GC and HPLC in actual hot fields in the market, about new developments in instrumentation for GC and HPLC, data evaluation software, columns, and consumables for analysis and sample preparation, and about hyphenation with mass spectrometry and other detection systems. The magazine also accepts more elaborate papers about progress in separation science and tutorial style manuscript explaining new techniques without too much depth to the readership of Chromatography Today. 

The editor of Chromatography Today has asked me to arrange a focus issue on CE and CE-MS. A number of my peers have contributed to the focus issue. The focus manuscripts will be part of the digital issue of Chromatography Today which you will find here soon (March issue).

My contribution to the issue deals with iCIEF. I have intended to provide a tutorial contribution painting the concept of iso-electrofocusing from flat gel plate to capillary formate and more recently to the instrumental implementation in imaged capillary isoelectric focusing. I hope the manuscript will interest you.

I have made it available from this website for registered visitors from here (be aware that depending on your browser settings, the document goes directly to your download folder and does not open). If you have registered on my website please click the button "registered visitors" and enter your credentials. If you have forgotten your credentials or want to register please use the button "contact me" also on the top of the page.


It is my pleasure to announce the publication by Agilent Technologies of a 100-page guidebook on CE/MS. It is intended as a resource for novices and practitioners of CE/MS and has a focus coverpage CE MS Guidebookon the Agilent CE/MS product and applications.

It is the intention of this guidebook to help novices to step into the practical application of CE/MS, and to provide current users with relevant information on method development and on diagnosis of practical problems. Towards this goal, the booklet is divided into two parts:

Part 1: Concepts of CE/MSDescription of the concept of CE/MS coupling with detailed emphasis to help understand the technique and to choose proper starting conditions. This part is my contribution.

Part 2: Practice of CE/MS Detailed description of the practice according to recommendations of Agilent that help to set up the CE instrument, the interface, the MS, and to provide current users with relevant information on method development and on the diagnosis of practical problems. Also, detailed guidelines to do system performance verification will be given. This second part is contributed by Agilent specialists mentioned on page 10 of the booklet. 

Leading experts in the field of CE/MS, Dr. Julie Schappler of Geneva University and Prof. Dr. Christian Neusüß have contributed with forewords.

The booklet is highly recommended especially for those who are considering applying CE/MS in their research. A click on the cover page will allow you to access the booklet from the Agilent website.


Page 1 of 3